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Changes in the expression of TGF-β1 and phenotype marker mRNAs in rat mesangial cells (MCs) with high glucose. Quiescent MCs from Wistar Kyoto rats were incubated with 25 mM glucose for 6 to 48 h. Total RNA was extracted, and the abundance of TGF-β1 (A), osteopontin (B) and αSMA (C) mRNAs was evaluated by real-time polymerase chain reaction analysis. Relative gene expression was analyzed by the comparison to GAPDH mRNA. Data are the mean ± SEM (n = 6). * p < 0.05 versus 0 h.
