Figure 3.

Morphological changes in the glomerulus of diabetic rats at 3 months after injection of streptozotocin (STZ). Diabetes was induced in male Wistar rats by single intraperitoneal injection of 60 mg/kg body weight of STZ. Control rats were intraperitoneally injected with 1 mL of 0.1% acetic acid. Three-millimeter-thick paraffin sections of removed renal cortex were stained with hematoxylin and eosin (HE) or Masson’s trichrome stain. The kidney sections were incubated with anti-podocin and then incubated with Alexa Fluor 488 anti-rabbit IgG. Scale bar indicates 25 µm.