Figure 6.

Effects of pyrrole-imidazole (PI) polyamide targeting TGF-β1 on the expression of TGF-β1 protein in kidney from streptozotocin (STZ) diabetic rats. Diabetes was induced in male Wistar rats by single intraperitoneal injection of 60 mg/kg body weight of STZ. Control rats were intraperitoneally injected with 1 mL of 0.1% acetic acid. Five milligrams of PI polyamide targeting TGF-β1 or Mismatch polyamide dissolved in 1 mL of 0.1% acetic acid was intraperitoneally injected twice a week for 3 months, and then the rats were sacrificed to remove their kidneys. Expression of TGF-β1 proteins was assessed by Western blot analysis. Data are the mean ±SEM (n = 3). * p < 0.05, ** p < 0.01 between indicated columns.