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Experimental protocol of the effects of pyrrole-imidazole (PI) polyamide targeting TGF-β1 on renal injury in streptozotocin (STZ) diabetic rats. STZ-induced diabetic rats were intraperitonially injected with 1 mL of 0.1% acetic acid as Control rats. Five milligrams of PI polyamide to TGF-β1 or Mismatch polyamide dissolved in 1 mL of 0.1% acetic acid was intraperitonially injected twice a week for 3 months, and then the rats were sacrificed to remove their kidneys.
