Table 2.
Effect of chrysin on glutathione-related enzymes’ activity, protein level and vitamin C level in the lenses of type 1 diabetic rats.
| Parameter | C | DM | CHR50 | CHR100 |
|---|---|---|---|---|
| GPx (nmol/min/mg of protein) | 1.19 ± 0.05 a | 1.24 ± 0.13 a | 1.06 ± 0.12 b | 1.17 ± 0.10 ab |
| GR (nmol/min/mg of protein) | 0.51 ± 0.19 NS | 0.33 ± 0.10 NS | 0.48 ± 0.22 NS | 0.32 ± 0.13 NS |
| G6PD (U/mg of protein) | 0.040 ± 0.006 NS | 0.029 ± 0.011 NS | 0.037 ± 0.017 NS | 0.035 ± 0.012 NS |
| Total protein (mg/g of the lens) | 563.2 ± 99.7 NS | 536.4 ± 123.5 NS | 560.5 ± 58.8 NS | 562.3 ± 187.9 NS |
| Soluble protein (mg/g of the lens) | 443.8 ± 25.2 a | 408.5 ± 39.2 b | 451.3 ± 13.7 a | 445.0 ± 19.7 a |
| Vitamin C (µg/g of the lens) | 7.69 ± 0.14 a | 7.17 ± 0.06 b | 7.27 ± 0.55 b | 7.40 ± 0.14 ab |
Results are presented as arithmetical means ± SD. The letters in the superscripts indicate statistical significances (one-way ANOVA followed by Fisher’s LSD test). The same letter(s) (a,b) in individual rows indicate no significant differences of values in measured parameters at p < 0.05; NS—not significant. C—control, non-diabetic rats; DM—control, type 1 diabetic rats; CHR50—type 1 diabetic rats treated by gavage with chrysin at a dose of 50 mg/kg for 28 days; CHR100—type 1 diabetic rats treated by gavage with chrysin at a dose of 100 mg/kg for 28 days; GPx—glutathione peroxidase; GR—glutathione reductase; G6PD—glucose-6-phosphate dehydrogenase.