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. 2020 Feb 3;9(2):132. doi: 10.3390/antiox9020132

Figure 2.

Figure 2

Dose- and time-dependent cell metabolic activity. Human Umbilical Vein Endothelial Cells (HUVECs) were cultured for 2 h (a) and 24 h (b) in the presence of increasing concentrations of total polyphenol content (TPC) from CE or CHE (5, 10, 25, 50, and 100 µg GAE/mL). Cell metabolic activity was determined by WST-1 colorimetric assay and expressed as metabolic activity percentage compared to control (untreated cells). Graphical data are represented as mean ± SD of three separate experiments run in triplicate. (*** p < 0.005, **** p < 0.0001 vs. control).