Figure 1.

Relative activity of Citrus reticulata O-methyltransferase 2 (CrOMT2) against four representative flavonoids and caffeic acid in vitro. The recombinant CrOMT2 purified from Escherichia coli was incubated with a number of phenolic substrates, and the relative activity against different substrates was estimated. The methylation sites identified for substrates are indicated in red in their structural formula. ND represents “not detectable”. R- in eriocitrin represents rutinoside and N- in neoeriocitrin represents neohesperidoside. The data are shown as means ± SE (n = 3).