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. 2020 Feb 15;25(4):858. doi: 10.3390/molecules25040858

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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HPLC analysis of products catalyzed by Citrus reticulata O-methyltransferase 2 (CrOMT2). The methylated products produced by the recombinant CrOMT2 were identified by comparison of the retention times and MS/MS data with corresponding standards (see Supplementary Figure S3 for the MS/MS data of standards). HPLC chromatograms of enzyme reactions with luteolin (A), tricetin (B), baicalein (C), quercetin (D), myricetin (E), quercetin 3-methyl ether (F), kaempferol (G), and eriodictyol (H) are indicated. Substrates catalyzed by CrOMT2 indicated in red; substrates incubated with empty vector (pET32a) indicated in blue, commercial standards of the methylated products indicated in black. Lut: luteolin; Chr: chrysoeriol; Tri: tricetin; Bai: baicalein; Bai 7-OMe: baicalein 7-methyl ether; Que: quercetin; Isor: isorhamnetin; Myr: myricetin; Syr: syringetin; Que 3-OMe: quercetin 3-methyl ether; Que 3,3’-diOMe: quercetin 3,3’-dimethyl ether; Kae: kaempferol; Isok: isokaempferide; Eri: eriodictyol; Hom: homoeriodictyol.