Figure 5.

Phylogenetic tree of Citrus reticulata O-methyltransferase 2 (CrOMT2) and selected plant caffeic acid O-methyltransferases (COMTs) at the protein level. The preferred substrates and methylation positions are indicated for each protein. Percent bootstrap values (n = 1000) greater than 70 are shown in the middle of each branch. The evolutionary history was analyzed using the neighbor-joining method with SaDMPM from Streptomyces alboniger acting as the outgroup. Two COMTs from citrus are indicated with arrows. The small clade including CrOMT2 from C. reticulata is boxed in black. To avoid ambiguity, CrOMT2 from Catharanthus roseus was marked with a triangle. See Supplementary Table S3 for UniProt entries and species of the OMTs.