Figure 1.
Multiplication coefficient (MC) for non-metallicolous (A), calamine (B) and serpentine (C) ecotypes of Silene vulgaris calculated separately for four and eight weeks of cultivation on media enriched with different heavy metal (HMs) concentrations. Values are means ± SD. Means indicated by the same letters (a–c) within each cultivation time point show statistically insignificant differences according to one-way ANOVA and post-hoc Fisher’s test at p < 0.05. MC represents the ratio of the newly regenerated shoots to initially used explants. Control means the medium without the addition of Zn, Pb, Cd ions; 1× HMs—medium enriched with 714.3 μM ZnSO4, 3.0 μM Pb(NO3)2, and 16.4 μM CdCl2; 2.5× and 5× HMs mean, respectively, 2.5- and 5.0-times higher Zn, Pb, Cd concentration than the first one (1× HMs).