Figure 3.

The high-throughput sequencing of 16S RNA in colonic feces of piglet. Con, the basal diet without antibiotics, tributyrin, oregano or methyl salicylate; AB diet, the basal diets supplemented with an antibiotic compound, which provides the diet with 120 mg oxytetracycline-calcium and 16 mg enduracidin per kilogram; OT and MT diets, the basal diet supplemented with tributyrin plus oregano (1.4 g and 0.6 g per kilogram diet) and tributyrin plus methyl salicylate (1.4 g and 0.6 g per kilogram diet), respectively. (a) Comparison of the phylum level between the 4 groups. (b) The relative ratio of the Firmicutes to Bacteroidetes. (c) The relative abundance of Proteobacteria. (d) Comparison of the abundance index (Chao1) between the 4 groups. (e) Comparison of the diversity index (Shannon) between the 4 groups. Error bars represent standard error of the mean. The p-values are based on Mann–Whitney tests. The statistical significance was the same when we used the unpaired t-test to log-transformed data. * Differences were considered significant at p < 0.05 (n = 6).