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Effect of Epiafzelechin from C. fistula on the generation of intracellular reactive oxygen species (ROS) in MG-63 cells (24 h). 2′,7′-dichlorofluorescein diacetate (DCFH-DA) was used to stained the cells, 20,000 events were acquired and the gated population analyzed using flow cytometer. M1 represents intact cell population and M2 represents cells with accumulation of intracellular ROS. * indicates a statistically significant difference at (p ≤ 0.05).
