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. 2020 Feb 5;10(2):248. doi: 10.3390/ani10020248

Figure 3.

Figure 3

Effect of NOS inhibitors and substrate on ovarian expression of PTEN (A), p-AKT (B), AKT (C), p-FoxO3a (D), and FoxO3a (E) in neonatal rats. Animals in the control, L-NAME, SMT, L-Arg, or L + S (L-NAME plus SMT) groups were subcutaneously hypodermically injected with PBS, L-NG-Nitroarginine Methyl Ester (40 mg/kg), S-Methylisothiourea (10 mg/kg), L-NG-Nitroarginine Methyl Ester (40 mg/kg) and S-Methylisothiourea (10 mg/kg), or L-arginine (50 mg/kg) for five days (PD5) from the first day of birth (PD1). β-tubulin was used as an internal control, wherein the signal intensity was plotted as the ratio of target protein to β-tubulin. Bars with different letters denote significant differences (p < 0.05).