Figure 1.

The experimental procedure and time table of the glass capillary immunoassay on autoantibodies. After the capillary was modified with the p53 antigen, the samples containing the anti-p53 antibody were transferred into the capillary, incubated for 20 min, and then washed three times to remove nonspecific antibody binding. Add the detection antibody into the capillary, incubate for 20 min, and then wash three times to remove the excess detection antibody; and finally, add the chemiluminescence substrate and record the result with a cellphone.