Figure 3.

Optimization of the p53 antigen concentration for capillary glass modification. The glass capillaries were modified with p53 antigen at various concentrations of 16.7, 8.35, 4.18, 2.09, and 1.05 ng mL⁻¹ and used for the immunoassay. The experiment was performed at fixed anti-p53 antibody and HRP-conjugated anti-mouse IgG concentrations with triplicates. The result indicates that the slope of the chemiluminescence intensity curve turns flat between p53 antigen concentrations of 8.36 and 16.7 ng µL⁻¹.