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Average per residue root mean square fluctuation (RMSF) computed from Cα atoms: (a) S108N, (b) N51I_S108N, (c) C59R_S108N, (d) N51I_C59R_S108N, (e) C59R_S108N_I164L, and (f) N51I_C59R_S108N_I164L. Color key: black: WT holo, green: WT complexed with pyrimethamine, magenta: mutant holo, blue: mutant complexed with pyrimethamine.
