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. 2020 Jan 22;12(2):291. doi: 10.3390/nu12020291

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Dietary vitamin D3 manipulation does not affect Th17 cells in the Act1-/- mouse. (A) Splenic Th17 cells (CD3+CD4+RORγt+) and (B) IL-17A production by splenic Th17 cells stimulated with PMA and ionomycin. (C) IL-1α, IL-6, and IL-17A cytokine profile after 9 weeks of dietary vitamin D3. (D,E) Association between splenic circulating Th17 cells and serum levels of IL-6 (D) and IL-17A (E). Each symbol represents an individual mouse. In (A,B), the mean of each group is represented by a horizontal line, and in (D,E) a best fit line is shown.