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. 2020 Jan 22;12(2):291. doi: 10.3390/nu12020291

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Memory B cells are specifically affected by vitamin D3 restriction. Splenocytes were analyzed for populations of (D) total B220+ B cells, (A–C) transitional B cells—T1 (B220+AA4.1+IgM+CD23-), T2 B220+AA4.1+IgM+CD23+), and T3 (B220+AA4.1+IgM-CD23+), (E) germinal center B cells (B220+IgM-GL7+CD38^low), (F) memory B cells (B220+IgM-CD38^hiGL7-), (G) plasmablasts (B220-CD138+IgM+) and (H) plasma cells (B220-CD138+IgM-). (I) Serum IL-10 levels. Each symbol represents an individual mouse, and the mean of each group is represented by a horizontal line. * p < 0.05, ** p < 0.01; unpaired t-test with Welch’s correction. GC = germinal center.