. 2020 Feb 24;25(4):996. doi: 10.3390/molecules25040996

Table 3.

IC₅₀ and selectivity index of C. verum extracts.

C. verum Extracts	Parasites	IC₅₀ (µg/mL) ^a		EC₅₀ (µg/mL) ^b			Selective Indices ^c
C. verum Extracts	Parasites	IC₅₀ (µg/mL) ^a	MDBK	NIH/3T3	HFF	MDBK	NIH/3T3	HFF
AECV	B. bovis	23.1 ± 1.4	440 ± 10.6	816 ± 12.7	914 ± 12.2	19	35.3	39.6
	B. bigemina	56.6 ± 9.1	440 ± 10.6	816 ± 12.7	914 ± 12.2	7.8	14.4	16.1
	B. divergens	33.4 ± 2.1	440 ± 10.6	816 ± 12.7	914 ± 12.2	13.2	24.4	27.2
	B. caballi	40.3 ± 7.5	440 ± 10.6	816 ± 12.7	914 ± 12.2	10.9	20.2	22.7
	T. equi	18.8 ± 1.6	440 ± 10.6	816 ± 12.7	914 ± 12.2	23.4	43.4	48.6
EAECV	B. bovis	40.1 ± 8.5	376 ± 11.2	610 ± 7.7	790 ± 12.4	9.4	15.2	19.7
	B. bigemina	55.6 ± 1.1	376 ± 11.2	610 ± 7.7	790 ± 12.4	6.8	11	14.2
	B. divergens	45.7 ± 1.9	376 ± 11.2	610 ± 7.7	790 ± 12.4	8.2	13.3	17.3
	B. caballi	50.2 ± 6.2	376 ± 11.2	610 ± 7.7	790 ± 12.4	7.5	12.2	15.7
	T. equi	61.5 ± 5.2	376 ± 11.2	610 ± 7.7	790 ± 12.4	6.1	9.9	12.8

^a Half-maximal inhibitory concentration of extracts on piroplasm parasites in vitro. ^b Half-maximal effective concentration of extracts on cell lines. The values were determined from the dose-response curve using non-linear regression (curve fit analysis). The values are the means of triplicate experiments. ^c Ratio of the cell lines EC₅₀ to the parasite IC₅₀. High numbers are favorable.