Table 3.
C. verum Extracts | Parasites | IC50 (µg/mL) a | EC50 (µg/mL) b | Selective Indices c | ||||
---|---|---|---|---|---|---|---|---|
MDBK | NIH/3T3 | HFF | MDBK | NIH/3T3 | HFF | |||
AECV | B. bovis | 23.1 ± 1.4 | 440 ± 10.6 | 816 ± 12.7 | 914 ± 12.2 | 19 | 35.3 | 39.6 |
B. bigemina | 56.6 ± 9.1 | 440 ± 10.6 | 816 ± 12.7 | 914 ± 12.2 | 7.8 | 14.4 | 16.1 | |
B. divergens | 33.4 ± 2.1 | 440 ± 10.6 | 816 ± 12.7 | 914 ± 12.2 | 13.2 | 24.4 | 27.2 | |
B. caballi | 40.3 ± 7.5 | 440 ± 10.6 | 816 ± 12.7 | 914 ± 12.2 | 10.9 | 20.2 | 22.7 | |
T. equi | 18.8 ± 1.6 | 440 ± 10.6 | 816 ± 12.7 | 914 ± 12.2 | 23.4 | 43.4 | 48.6 | |
EAECV | B. bovis | 40.1 ± 8.5 | 376 ± 11.2 | 610 ± 7.7 | 790 ± 12.4 | 9.4 | 15.2 | 19.7 |
B. bigemina | 55.6 ± 1.1 | 376 ± 11.2 | 610 ± 7.7 | 790 ± 12.4 | 6.8 | 11 | 14.2 | |
B. divergens | 45.7 ± 1.9 | 376 ± 11.2 | 610 ± 7.7 | 790 ± 12.4 | 8.2 | 13.3 | 17.3 | |
B. caballi | 50.2 ± 6.2 | 376 ± 11.2 | 610 ± 7.7 | 790 ± 12.4 | 7.5 | 12.2 | 15.7 | |
T. equi | 61.5 ± 5.2 | 376 ± 11.2 | 610 ± 7.7 | 790 ± 12.4 | 6.1 | 9.9 | 12.8 |
a Half-maximal inhibitory concentration of extracts on piroplasm parasites in vitro. b Half-maximal effective concentration of extracts on cell lines. The values were determined from the dose-response curve using non-linear regression (curve fit analysis). The values are the means of triplicate experiments. c Ratio of the cell lines EC50 to the parasite IC50. High numbers are favorable.