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. 2020 Jan 23;10(2):197. doi: 10.3390/ani10020197

Table 1.

Primer pairs of the studied candidate genes.

Gene Name Gene ID Allele 2 Primer Sequence Product Size (bp) Annealing Temperature (°C)
LEP 1 ENSOARG00000002407 T/G(V181L) F:AGGAAGCACCTCTACACTC
R:CTTCAAGGCTTCAGCACC
471 53
IGF1 ENSOARG00000015856 G/A F:GTTCTGGAATGGCAGGTTTG
R:GCCACTGTCTTTGGATTTTCTC
570 60
DGAT1 ENSOARG00000014070 T/C F:ACTGTGCTTCAGGGTGTCGG
R:GAGTGATGGACTCTAGGAGGAAGG
429 60
PRL ENSOARG00000009137 A/G F:TGGAATTTAGATGACAAGCAACTG
R:AATTGGTGGCTCAAGTGGTG
745 63
CSN1S2 ENSOARG00000010683 A/C F:CCCTGAAGGAATCTGCTGAAG
R:AGCCAAGCAAAATGATATAGAAGC
855 63
GHR ENSOARG00000008837 C/T(P448S) F:TGATGACCCTGATGAGAAGACTG
R:TTTTGTTCAGTTGGTCTGTGCTC
857 63
GHRHR ENSOARG00000007636 C/T F:TTGTTCTTGGAGGTGAGGACTG
R:AACACGGGTGGCTCTCTTG
759 63
STAT5A ENSOARG00000000809 G/A F:GGGTGCATACAGGACAGTGC R:CCAGTCTCTGGCTTTCCCAA 446 60

1 primer design according to [32]; 2 If present, consequences at the protein level are shown.