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. 2020 Jan 29;10(2):220. doi: 10.3390/ani10020220

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemical techniques: (a) Degenerated cardiomyocytes (thick arrows) present depletion of cardiac troponin I (cTnI), compared to the normal cardiomyocytes (arrow heads). 10×. Inset: Detail of the intrafibrillar depletion (thick arrows) of cTnI in the injured cardiomyocytes, in comparison with normal cardiomyocytes (thin arrows); 60×, anti-troponin I; (b) Necrotic cardiomyocytes (thick arrows), close to the blood vessels (*) demonstrate depletion of cardiac troponin C (cTnC), compared to the normal cardiomyocytes (arrow heads); 20×. Inset: Detail of intrafibrillar depletion of cTnC with intense immunolabelling in the contraction band necrosis (thick arrows); 60×, anti-troponin C; (c) Degenerated cardiomyocytes (thick arrows), show intrafibrillar depletion of myoglobin, compared to the normal cardiomyocytes (thin arrows); 10×. Inset: Detail of the intrafibrillar depletion (thick arrows) of myoglobin in the injured cardiomyocytes, in comparison with normal cardiomyocytes (thin arrows); 20×, anti-myoglobin; (d) Expression of fibrinogen, in the necrotic myocytes, mainly in area next to (thin arrows) the contraction band necrosis (thick arrows). Presence of fibrinogen in the blood vessels (arrow head); 20×. Inset: Detail of the immunolabelling of fibrinogen, in the injured myocytes, in the zone near to (thin arrows) the contraction band necrosis (thick arrows). Fibrinogen inside the blood vessels (arrow heads); 60× anti-fibrinogen.