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. 2020 Feb 23;25(4):994. doi: 10.3390/molecules25040994

Figure 6.

Figure 6

EDG and DC inhibited FcεRI-mediated calcium influx and degranulation. Cells treated with various concentrations of EDG and DC under serumfree conditions. (A) Cells incubated with Fura 2-AM and stimulated with CRA-1 to determine calcium influx. (B) To examine histamine content in the medium, cells were stimulated with CRA-1, and supernatants were treated with o-phthalaldehyde (OPA). These cells were determined via a spectrofluorometric method. Each value expressed as mean ± SD of three different experiments. # p < 0.05 vs. non-treated group; * p < 0.05 vs. CRA-1-treated group.