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. 2020 Feb 11;25(4):772. doi: 10.3390/molecules25040772

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Multiple alignments of Nitrile-specifier Protein (NSP) from broccoli homolog organisms (Q9SDM9, M4FIJ4, A0A078G479, and A0A0D3AD54), generated by means of Clustal W. Gaps and conservation residues were denoted in dashes (-) and asterisks (*), respectively. In addition, colon (:) and period (.) indicate strong and weak properties conservation between groups, respectively. The color code represents the physicochemical property of the amino acid (red: small or hydrophobic, blue: acidic; magenta: basic; green: hydroxyl or sulfhydryl or amine; grey: unusual amino/imino acids).