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. 2020 Mar 6;37(6):846–859. doi: 10.1089/neu.2019.6526

FIG. 6.

FIG. 6.

Blocking KCC2 or BDNF activity during exercise prevents the activity-dependent increase in KCC2 levels in lumbar motoneuronal membrane. (A) Digital images showing KCC2 expression in the membrane around the motoneuronal soma (white arrows) and also in dendrites in the ventral horn of the lumbar enlargement. (B) Quantification of the integrated density of KCC2 around the motoneuronal membrane reveals that exercise increases KCC2 immunoreactivity (p < 0.001) and that blocking KCC2 or BDNF activity prevents this activity-dependent increase after SCI (p < 0.001). Data are presented as mean ± SEM. Kruskal-Wallis ANOVA on ranks (p < 0.001) followed by Dunn's method, n > 15 motoneurons/group. ***P < 0.001, scale bar = 25 μm. ANOVA, analysis of variance; BDNF, brain-derived neurotrophic factor; SCI, spinal cord injury.