Figure 6.

MMP-1 expression and galactosidase staining analyses of UV + CPO treated cells. (A) Western blot for MMP-1 protein. The level of MMP-1 and β-actin protein in lysates were determined by Western blotting using HRP-labeled anti-rabbit IgG antibody. Band intensities were measured using an imaging densitometer. The relative levels of MMP-1 protein were calculated versus β-actin protein. Two to three dishes per group were combined for western blotted analysis and samples were assayed in duplicate. (B) Galactosidase staining analyses. After incubation with galactosidase staining solution at 37 °C, color intensity in the cells were observed at 400× magnification under a microscope. Arrows indicated a X-gal stained cell. Two to three dishes per group were combined for western blotted analysis and samples were assayed in duplicate. Data are reported as the means ± SD. * p < 0.05 compared to the No group. # p < 0.05 compared to the UV + Vehicle group. Abbreviations: LCPO, low concentration CPO; MCPO, medium concentration CPO; HCPO, high concentration CPO; GEGR, gallotannin-enriched extract of Galla Rhois.