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. 2020 Feb 22;25(4):989. doi: 10.3390/molecules25040989

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Skin phenotypical analyses of UV + CPO treated mice. (A) Wrinkle formation on the skin was measured by replica grading after 4 weeks of CPO treatment. Wrinkles were assessed as described by Bissett et al. [19] (grade 0, no wrinkles; grade 1, a few shallow wrinkles; grade 2, some wrinkles; grade 3, several deep wrinkles). (B) TEWL, skin hydration, and erythema and melanin indices of dorsal skins were analyzed in triplicate as described in Materials and Methods. Three to five mice per group were used and two different dorsal skin areas per mouse were assayed in duplicate. Data are reported as the means ± SD. * p < 0.05 compared to the No group. # p < 0.05 compared to the UV + No group. Abbreviations: LoCPO, low amount CPO; HiCPO, high amount CPO.