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. 2020 Feb 22;25(4):989. doi: 10.3390/molecules25040989

Figure 9.

Figure 9

Level of galactosidase and MMP-3 mRNA. (A) Galactosidase staining analyses. The morphological features of cells stained with X-gal in dimethylformamide solvent at 37 °C, were observed in skin tissues at 400× magnification. Three to five mice per group were used and galactosidase levels were determined in duplicate for each mouse. (B) Expression level of MMP-3 mRNA. Levels of MMP3 in skin total mRNA were determined by quantitative real-time (qRT)-PCR using specific primers. mRNA levels were calculated with respect to β-actin mRNA (endogenous control). Three to five mice per group were used and PCR was conducted in duplicate. Data are reported as the means ± SD. * p < 0.05 compared to the No group. # p < 0.05 compared to the UV + No group. Abbreviations: LoCPO, low amount CPO; HiCPO, high amount CPO.