Table 1.
Primers used for real-time quantitative PCR.
| Genes | Accession No. | Primers | Sequences (5′-3′) |
|---|---|---|---|
| E-cadherin | NM_001163060.1 | Forward | GAAGGAGGTGGAGAAGAGGAC |
| Reverse | AGAGTCATAAGGTGGGGCAGT | ||
| Occludin | NM_001163647.2 | Forward | AGAGTCATAAGGTGGGGCAGT |
| Reverse | CGCCCGTCGTGTAGTCTGTC | ||
| ZO-1 | XM_005659811.1 | Forward | TACCCTGCGGCTGGAAGA |
| Reverse | GGACGGGACCTGCTCATAACT | ||
| Integrin | NM_213968.1 | Forward | GCAGTTTCAAGGTCAAGATGG |
| Reverse | AGCAGGAGGAAGATGAGCAG | ||
| β-actin | XM_003124280.3 | Forward | GGATGCAGAAGGAGATCACG |
| Reverse | ATCTGCTGGAAGGTGGACAG |
ZO-1, zonula occludens-1.