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. 2020 Feb 24;10(2):367. doi: 10.3390/ani10020367

Figure 5.

Figure 5

Inhibin A alters the stages of GCs cycle: Flow cytometric analysis of GCs cultured under the treatment of inhibin A (100 ng/mL) and the corresponding control group. The Y axis shows the analyzed cell counts while the X axis indicates the DNA content of cells stained by PI staining (A). mRNA expression of PCNA (B) and CyclineB1 (C) in GCs cultured under different concentrations of inhibin A (20, 50, and 100 ng/mL) and the corresponding control (0 ng/mL). GADPH was used as a reference gene. The results are expressed as the mean ± SEM, n = 3. The bars labeled with completely different letters indicate a significant difference, p < 0.05.