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. 2020 Feb 24;10(2):367. doi: 10.3390/ani10020367

Figure 6.

Figure 6

Flow cytometric analysis of GCs cultured under the treatments of different inhibin A doses (50 and 100 µg/mL). The Y axis shows the analyzed cell counts for apoptosis while the X axis indicates the doses of inhibin A DNA content of cells stained by PI staining. The analyzed cell counts for apoptosis are indicated on the Y axis, and the doses of inhibin A are indicated on the X axis (A). Data shown as means ± SEM, n = 3, p < 0.05. mRNA expression of INHβA (B) and pro-apoptotic genes BAX (C) and Caspase-3 (D) in GCs cultured at different concentrations of inhibin A (20, 50, and 100 ng/mL) and the corresponding control (0 ng/mL). GADPH was used as a reference gene. The results are expressed as the mean ± SEM, n = 3. The bars labeled with completely different letters indicate a significant difference, p < 0.05.