Table 4.
Values of vitamins, homocysteine, and inflammatory markers, separated by the Methylenetetrahydrofolate reductase MTHFR genotypes, groups and pre and post-diet intervention with folate.
| Group 1 95 µg/Day | |||||||||
| Pre | Post | p-value | Pre | Post | p-value | Pre | Post | p-value | |
| CC | CC | CT | CT | TT | TT | ||||
| Folate (μg) | 149.0 ± 14.43 | 292.0 ± 65.24 | 0.0004 * | 170.0 ± 50.43 | 254.0 ± 85.27 | 0.0910 | 154.0 ± 53.84 | 316.0 ± 63.06 | 0.0003 * |
| FA (μg/mL) | 13.18 ± 4.08 | 14.88 ± 3.79 | 0.1318 | 13.32 ± 3.97 | 16.4 ± 4.95 | 0.0011 * | 15.00 ± 4.60 | 22.62 ± 6.30 | 0.0249 * |
| Vit. B12 (μg/mL) | 268.6± 92.63 | 271.0 ± 92.16 | 0.3185 | 345.5 ± 179.14 | 355.2 ± 202.59 | 0.7991 | 299.8 ± 118.65 | 273.6 ± 83.60 | 0.3162 |
| Hcy (μmol/L) | 13.61 ± 13.10 | 7.83 ± 2.51 | 0.2408 | 10.98 ± 6.11 | 9.86 ± 4.43 | 0.6812 | 19.33 ± 12.75 | 14.91 ± 10.40 | 0.4603 |
| TNF-α (pg/mL) | 5.80 ± 8.33 | 4.45 ± 3.54 | 0.6489 | 9.52 ± 8.63 | 2.84 ± 2.09 | 0.0517 | 3.10 ± 2.34 | 4.30 ± 6.99 | 0.5959 |
| IL-6 (pg/mL) | 4.26 ± 5.10 | 4.48 ± 2.29 | 0.9184 | 5.16 ± 1.60 | 2.82 ± 2.96 | 0.0685 | 3.85 ± 4.39 | 3.29 ± 3.07 | 0.8310 |
| IL-1β (pg/mL) | 3.50 ± 4.44 | 3.85 ± 1.78 | 0.8280 | 5.43 ± 4.50 | 1.55 ± 1.79 | 0.0224* | 2.71 ± 2.34 | 3.69 ± 4.58 | 0.6713 |
| Group 2 191 µg/Day | |||||||||
| Pre | Post | p-value | Pre | Post | p-value | Pre | Post | p-value | |
| CC | CC | CT | CT | TT | TT | ||||
| Folate (μg) | 132.0 ± 32.73 | 352.0 ± 73.07 | 0.0000 * | 128.0 ± 41.17 | 410.0 ± 372.35 | 0.0000 * | 184.0 ± 34.54 | 376.0 ± 68.32 | 0.0000 * |
| FA (μg/mL) | 12.94 ± 5.29 | 22.21 ± 7.26 | 0.0033 * | 13.48 ± 1.61 | 18.11 ± 3.10 | 0.0016 * | 16.52 ± 5.11 | 25.48 ± 4.35 | 0.0046 * |
| Vi. B12 (μg/mL) | 251.8 ± 113.13 | 237.5 ± 52.69 | 0.7174 | 261.2 ± 139.79 | 258.0 ± 113.03 | 0.8429 | 246.3 ± 52.71 | 228.6 ± 78.15 | 0.6492 |
| Hcy (μmol/L) | 8.97 ± 1.52 | 7.60 ± 1.79 | 0.1207 | 9.91 ± 3.70 | 8.01 ± 2.27 | 0.2374 | 10.05 ± 1.66 | 7.11 ± 0.77 | 0.0005 * |
| TNF-α (pg/mL) | 7.73 ± 12.34 | 5.46 ± 9.38 | 0.5132 | 5.02 ± 4.59 | 1.66 ± 2.31 | 0.1614 | 6.78 ± 5.24 | 1.66 ± 0.53 | 0.0004 * |
| IL-6 (pg/mL) | 3.93 ± 4.70 | 3.63 ± 3.41 | 0.8980 | 3.86 ± 3.76 | 1.84 ± 2.03 | 0.2224 | 5.67 ± 0.93 | 0.66 ± 0.74 | 0.0000 * |
| IL-1β (pg/mL) | 3.17 ± 5.74 | 17.62 ± 1.63 | 0.2610 | 6.85 ± 5.82 | 3.01 ± 3.43 | 0.1565 | 5.32 ± 2.20 | 1.73 ± 2.47 | 0.0007 * |
* All values are expressed as mean and standard deviation (±SD). p-values refer to the Student’s t-test for paired sample mean differences. In all t statistics, the degree of freedom of the model was equal to 15. G1: 191 µg/day of folate; G2: 95 µg/day of folate. FA: folic acid (μg/mL); Vit. = Vitamin; IL- interleukin; TNF: tumor necrosis factor; Hcy: homocysteine; CC, cytosine-cytosine; CT, cytosine-thymine; TT, thymine-thymine.