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. 2020 Jan 27;12(2):336. doi: 10.3390/nu12020336

Figure 1.

Figure 1

Effects of ethanol extract of Spergularia marina (SME) on proliferation and differentiation of 3T3-L1 cells. The 3T3-L1 pre-adipocytes were treated with SME (50, 100, and 200 μg/mL) for 48 h. Dimethyl sulfoxide (DMSO) was used as control. (A) Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (B) Adipocyte differentiation was accessed by glycerol-3-phosphate dehydrogenase (GPDH) measurement (C) Triglyceride (TG) content was determined by manual methods. (D) Lipoprotein lipase (LPL) activity was assayed using the Nilsson-Ehle and Schotz methods. Values are means ± SDs, n = 3. Data were analyzed by one-way ANOVA using Kruskal–Wallis analysis followed by Tukey’s post hoc test. Labeled means without a common letter differ, p < 0.05.