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. 2020 Jan 31;12(2):373. doi: 10.3390/nu12020373

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Hepatic expression of advanced glycation end-product (AGE) receptors. Mice were treated as described in Figure 1. Liver was collected and flash frozen for immunoblotting or fixed in formalin and paraffin embedded for immunohistochemical analysis. The liver sections were probed for the following: (A–C) Galectin-3 (red: Gal-3); (D–F) the receptor for advanced glycation end-products (green: RAGE). All images were acquired using a 20x objective, and a selected area was cropped and enlarged. Images are representative of at least replicate images captured per mouse in 8 to 12 mice per treatment group. Immunofluorescent images were semi-quantified, and immunoblot band densities were analyzed using ImageJ software and normalized to HSC70. Values represent means ± SEM. * p < 0.05; ** p < 0.001. Pair-fed saline (PF-S); Ethanol-fed saline (EF-S); Ethanol-fed synbiotic (EF-Syn).