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. Author manuscript; available in PMC: 2020 Mar 14.
Published in final edited form as: Biochem J. 2019 May 31;476(10):1521–1537. doi: 10.1042/BCJ20190182

Figure 4. Inhibition of BCKDH kinase in vivo does not impact maximal BCKA-supported mitochondrial respiration.

Figure 4.

(A) Schematic of experimental timeline with BT2. (B) Representative fluorescent trace depicting KMV-supported JNADH in alamethicin-permeabilized heart mitochondria from mice treated with DMSO or BT2. (C) Quantification of BCKDH and PDH activity from heart mitochondria of mice treated with DMSO or BT2. Data are expressed as a percentage of activity from DMSO-treated mice. (D) Relationship between JO2 and ΔGATP for KMV-energized heart mitochondria from DMSO and BT2-treated mice. Rates to the left of the dotted line correspond to mitochondria alone (‘mt’). Data are mean ± SEM, n = 4/group, *P < 0.05.