Figure 7.

Flow cytometry study for exosomes derived from MCF7, MDA-MB-231 and SKBr3 breast cancer cell lines attached on magnetic particles (MPs), being (Panel A) covalent immobilization of the exosomes on MPs (exosomes-MPs) and (Panel B) IMS of the exosomes on antiCD81-MPs, followed by indirect labeling with mouse antiCDX (5 µg mL⁻¹), (CDX being either CD9, CD24, CD44, CD54, CD63, CD81, CD326 or CD340 biomarkers) and antimouse-Cy5 (2 µg mL⁻¹). The concentrations of MPs and exosomes were set at 1 × 10⁶ MPs and 4 × 10⁹ exosomes per assay, respectively.