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. 2020 Mar 14;20:80. doi: 10.1186/s12935-020-01169-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Impact of circPITX1 knockdown on glycolysis and radioresistance of glioma cells. a–h U251 and LN229 cells were transfected with si-circPITX1 or si-NC. a QRT-PCR assay for the relative expression of circPITX1 in transfected glioma cells. b, c MTT assay for the viability of transfected glioma cells. d–f The glucose consumption, lactate production and ATP level in transfected U251 and LN229 cells. g, h Western blot assay for the levels of HIF-1α, HK2 and LDH-A in transfected U251 and LN229 cells. i, j Colony formation assay for the survival rate of U251 and LN229 cells transfected with si-circPITX1 or si-NC under X-ray radiation treatment (0 Gy, 2 Gy, 4 Gy, 6 Gy and 8 Gy). k, l Colony formation assay for the colony formation rate of U251 and LN229 cells transfected with si-circPITX1 or si-NC treated with 6 Gy of X-rays or not. *P < 0.05