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. 2020 Mar 14;20:80. doi: 10.1186/s12935-020-01169-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — MiR-329-3p was a target of circPITX1. a The binding sites between circPITX1 and miR-329-3p, as well as the mutant. b, c Dual-luciferase reporter assay for the luciferase activity in glioma U251 and LN229 cells cotransfected with circPITX1-WT or circPITX1-MUT and miR-329-3p or miR-NC. d, e QRT-PCR assay for the relative expression of miR-329-3p in glioma tissues and normal tissues, as well as in glioma U251 and LN229 cells and NHA. f Pearson correlation analysis for the correlation between the expression of circPITX1 and miR-329-3p in glioma tissues. R = − 0.574, P < 0.0001. g QRT-PCR assay for the relative expression of miR-329-3p in U251 and LN229 cells transfected with si-circPITX1 or si-NC. h QRT-PCR assay for the relative expression of miR-329-3p in U251 and LN229 cells transfected with pcDNA-circPITX1 or pcDNA-NC. *P < 0.05