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. 2020 Mar 14;20:80. doi: 10.1186/s12935-020-01169-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — MiR-329-3p directly targeted NEK2. a The binding sites between miR-329-3p and NEK2, as well as the mutant. b, c Dual-luciferase reporter assay for the luciferase activity in glioma U251 and LN229 cells cotransfected with NEK2 3′UTR-WT or NEK2 3′UTR-MUT and miR-329-3p or miR-NC. d QRT-PCR assay for the relative mRNA expression of NEK2 in glioma tissues and normal tissues. e Pearson correlation analysis for the correlation between the expression of miR-329-3p and NEK2 in glioma tissues. R = − 0.515, P < 0.0001. f Western blot assay for the protein level of NEK2 in glioma tissues and normal tissues. g QRT-PCR assay for the relative mRNA expression of NEK2 in glioma U251 and LN229 cells and NHA. h Western blot assay for the protein level of NEK2 in glioma U251 and LN229 cells and NHA. i QRT-PCR assay for the relative mRNA expression of NEK2 in U251 and LN229 cells transfected with miR-NC, miR-329-3p, miR-329-3p + pcDNA-NC or miR-329-3p + pcDNA-NEK2. j Western blot assay for the protein level of NEK2 in U251 and LN229 cells transfected with miR-NC, miR-329-3p, miR-329-3p + pcDNA-NC or miR-329-3p + pcDNA-NEK2. *P < 0.05