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. 2020 Mar 14;20:217. doi: 10.1186/s12885-020-6686-x

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© The Author(s). 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — ELN increases human colon cancer epithelial cell proliferation and invasion. Human colon cancer epithelial cells (Caco-2) were seeded onto plates coated with recombinant ELN protein and cultured in medium with (or without) ELN peptide. Control plates were coated with PBS only. a Cell proliferation was assessed after 6, 12, 24 and 48 h incubation by MTT assay. b A straight scratch was performed in each well and cancer cells invasion and migration were assessed by wound healing. c Wound area was assessed by measuring the wound closure size after 6 and 12 h post scratch, scale bar is 200 μm. n = 4. Results are mean ± SEM. *P < 0.05, ***P < 0.001 compared to cells cultured in normal medium on control plates. ††† < 0.001, compared to cells cultured in medium contain ELN peptide on control plates. #P < 0.05 compared to cells cultured in normal medium on ELN coated plates