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. 2020 Mar 14;20:39. doi: 10.1186/s12902-020-0515-8

Table 3.

Primers used for qRT-PCR assessment of gene expressions

Gene Primers Primer sequence Tm
Cox-1 Forward 5-GGTAACTGCTTAGGACCAGT-3 58
Reverse 5-ACTGTTCTCCGTACCTTCAC-3
Cox-2 Forward 5-TGTCATTCCAGAGTGCTGAG-3 58
Reverse 5-GGCTAACAAGGAGTTCAGCA-3
Beta-Actin Forward 5-GAT CTC CTT CTG CAT CCT GT-3′ 57
Reverse 5′-TGG GCA TCC ACG AAA CTA C- 3’