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. 2020 Mar 13;11:113. doi: 10.1186/s13287-020-01617-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — MSC-MVs mediate transfer of miR-100 to BLM-treated L2 cells. a qRT-PCR analysis of miR-100 expression in MSCs and MSC-MVs. b qRT-PCR analysis of miR-100 expression in NCI-transfected MSCs and miR-100I-transfected MSCs. c qRT-PCR analysis of miR-100 expression in MVs derived from NCI-transfected MSCs (NCI-MVs) and MVs from miR-100I-transfected MSCs (miR-100I-MVs). d The MVs derived from FAM (green)-miR-100-transfected MSCs were labeled with Dil (red) and co-cultured with rat type II alveolar epithelial cell line L2 (nuclei stained with Hoechst 33342, blue). The distribution and intensity of fluorescence were observed by confocal laser microscopy to analyze MV uptake by L2 cells. e qRT-PCR analysis of miR-100 expression in L2 cells in the groups of control, BLM, BLM+MVs, BLM+NCI-MVs, and BLM+miR-100I-MVs. Data are presented as the means ± SD (n = 3). **p < 0.01, vs. MSC; ^##p < 0.01, vs. NCI; ^$$p < 0.01, vs. NCI-MVs; ^&&p < 0.01, vs. control; ^@@p < 0.01, vs. BLM; ^%p < 0.05, vs. BLM+NCI-MVs