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. 2020 Mar 13;13:39. doi: 10.1186/s13041-020-00572-x

Fig. 3.

Fig. 3

Documenting glutamate release from multiple axonal boutons using linescan imaging mode. a Linescan image (left) depicting the iGluSnFR.WPRE.SV40 fluorescence time course in three axonal boutons (b5-b7, right; ROI as in Fig. 2) during afferent stimulation (five pulses at 20 Hz), with fEPSP monitoring (red trace). b Traces, ΔF/F0 signal time course recorded as shown in (a) (one-trial example).