Fig. 2.

Upregulation of miR-495 or HOXC6 silencing represses the TGF-β signaling pathway. The cells were treated with miR-495 mimic, miR-495 inhibitor, or si-HOXC6. a The miR-495 expression and mRNA levels of HOXC6, TGF-β, TGFβRI, TGFβRII, and Smad4 in cells detected by RT-qPCR. b, c, The protein levels of HOXC6, TGF-β, TGFβRI, TGFβRII, Smad2, and Smad4 in cells examined by Western blot analysis. *p < 0.05 vs. the blank group and the NC group. Data (mean ± standard deviation) were analyzed by independent sample t test. The experiment was repeated three times independently