Fig. 2.

Accuracies (y-axis) of classifying different types of reads for the V3-V5 primer pair at various top-hit identities (x-axis). Three types of reads, first (r1), directly joined (dj), and inside-out (io) reads, were classified to the genus level at different top-hit identities by six classification methods using MiSeq 2 × 300 bp data. The directly joined and inside-out reads were also compared to the corresponding rearranged references (djref and ioref) respectively for classification