Fig. 3.

lncARSR participates in activation of the IRS2/AKT pathway by blocking YAP1 phosphorylation. a LncARSR expression after alteration of lncARSR measured using RT-qPCR. b YAP1 expression and phosphorylation of YAP1 after alteration of lncARSR assessed by western blot analysis. c Co-localization of lncARSR and YAP1 after alteration of lncARSR detected by RNA FISH (×400). d Expression of IRS2, AKT and phosphorylation of AKT in NAFLD mice determined by western blot analysis. e Levels of IRS2, AKT and phosphorylation of AKT in oleate-treated HepG2 cells examined by western blot analysis. f IRS2 expression and phosphorylation of AKT after alteration of lncARSR and YAP1 detected by western blot analysis. *p < 0.05 against normal or control treatments; ^@p < 0.05 against sh-NC treatment; ^#p < 0.05 against oe-NC treatment ^&p < 0.05 against sh-lncARSR treatment; ^$p < 0.05 against oe-lncARSR treatment. The measurement data were descried as mean ± standard deviation. Unpaired t-test was used for comparison between two groups; one-way ANOVA for analyzing multiple groups. Experiments were performed three times