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. Author manuscript; available in PMC: 2020 Oct 15.
Published in final edited form as: Biochem J. 2019 Oct 15;476(19):2835–2850. doi: 10.1042/BCJ20190410

Figure 2. Canonical ASK1 targets are activated in platelets.

Figure 2.

Western blot analysis of lysates of human platelets stimulated with thrombin (0.1U/mL) for the indicated time. (A) Images of representative immunoblots of phosphorylation of ASK1, MKK3, MKK4, MKK6, MKK7 and p38 using phospho-specific antibodies. Phospho-blots were reprobed for their respective total (anti-ASK1, anti-MKKs, anti-p38) antibodies to ensure equal protein loading. (B) Quantification of band intensities of each MKKs from (A).