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. Author manuscript; available in PMC: 2020 Oct 15.
Published in final edited form as: Biochem J. 2019 Oct 15;476(19):2835–2850. doi: 10.1042/BCJ20190410

Figure 6. Platelet agonists activate ASK1 in a Ca2+-dependent manner.

Figure 6.

(A & B) Western blot analysis of human platelets pretreated with either DMSO or BAPTA/AM (50μM) before being stimulated with (A) PAR4-AP (100μM) or (B) convulxin (10ng/mL). (Ai & Bi) Images of representative immunoblots of phosphorylation of ASK1 and p38 using phospho-specific antibodies. Blots were reprobed with anti-ASK1 and anti-p38 antibodies to ensure equal protein loading. Quantification of the band intensities of (Aii & Bii) P-ASK1 and (Aiii & Biii) P-p38 from (Ai & Bi), ***P<0.001, ****P<0.0001, two-way ANOVA.