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. Author manuscript; available in PMC: 2020 Oct 15.
Published in final edited form as: Biochem J. 2019 Oct 15;476(19):2835–2850. doi: 10.1042/BCJ20190410

Figure 8. CIB1, but not Trx, associates with ASK1 in resting platelets.

Figure 8.

(A-C) Representative western blot images of immunoprecipitate of anti-ASK1 or normal rabbit IgG antibodies from resting and thrombin (1U/mL, used to elicit full dissociation of CIB1 from ASK1) stimulated platelet lysates and blotted for (A) Trx, (Bi) CIB1, and (Ci) TRAF6. Input is 5% of lysates used for immunoprecipitation. Blots were reprobed with anti-ASK1 antibody to ensure equal loading. (Bii) Quantification of the band intensity from (Bi); *P<0.05, Student’s t-test. (Cii) Quantification of the band intensity from (Ci); *P<0.05, **P<0.01, one-way ANOVA with Dunnett’s multiple comparisons test.