Skip to main content
. Author manuscript; available in PMC: 2020 Mar 14.
Published in final edited form as: Cell Rep. 2019 Jun 25;27(13):3988–4002.e5. doi: 10.1016/j.celrep.2019.05.086

Figure 5. MHV68 ORF Diversity.

Figure 5.

(A) Types of primary ORFs encoded by TRIMD transcripts.

(B) Locations of splice junctions within TRIMD transcripts.

(C) Transcripts containing ORF61 and its variants. The pink boxes in the splice junctions of ORF61-02, ORF61b-01, and ORF61a-01 represent a spliced-out upstream ORF. The overlapping transcripts with other primary ORFs are not displayed.

(D) Transcripts containing ORF10 and its variants. The overlapping transcripts with other primary ORFs are not displayed.

(E) RT-PCR using primers spanning ORF61 variant splice junctions. Representative of 3 biological replicates.

(F) RT-PCR using primers spanning ORF10 variant splice junctions. Representative of 3 biological replicates.

(G) Occurrence of ORF61 variant splice junctions in validation sample sets.

(H) Occurrence of ORF10 variant splice junctions in validation sample sets.