Table 2.
Experimental and predicted populations of in/out/tethered gates in the 20S CP with and without the 11S RP
| 20S CP:11S RP* | In | Out | In/out | Tethered | |
| 1:0 | Observed | 28.1 ± 0.8% | 71.9 ± 1.3% | 0.390 ± 0.013 | 0% |
| Expected | 28.6% | 71.4% | 0.4 | 0% | |
| 1:3 | Observed | 25.8 ± 0.5% | 61.3 ± 1.8% | 0.42 ± 0.02 | 12.9 ± 0.8% |
| Expected without allostery | 23.8 ± 1.4% | 59.0 ± 2.0% | 0.40 ± 0.02 | 17.2 ± 2.4% | |
| Expected with maximum allostery | 11.0 ± 1.8% | 71.8 ± 2.8% | 0.15 ± 0.03 | 17.2 ± 2.4% |
Observed populations are calculated from peak volumes, corrected for differential transverse relaxation. See Materials and Methods for details about concentrations used.
*
Concentrations of 20S CP and 11S RP are 22 and 65 μM, respectively. Note that there are two populations of 20S CPs, corresponding to the NMR invisible symmetric complex where both α7-rings are MII+M6A+S95C (46%), and a second 20S CP (54%) comprising one M1I+M6A+K66A+S95C α7-ring (NMR active) + one NMR inactive M1I+M6A+S95C α7-ring.